Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristicsReference or source
E. coli strains
 MC1061F lac 4
 BL21(DE3)F ompT(rB mB ) prophage λ carrying T7 RNA polymerase gene 39
V. cholerae strains
 TRH7000El Tor; Δ(ctxAB), wild type with respect to extracellular protein secretion 11
 Mut8TRH7000 with Kmr insertion in epsLgene 34
A. hydrophilastrains
 Ah65Wild type 4
 89AKmr insertion in exeL geneThis study
Plasmids
 pMMB207, pMMB208Broad-host-range cloning vectors; Cmr 8, 22
 pMMB532 epsM in pT7-5This study
 pMS49 epsL in pMMB208This study
 pMS55 exeL154epsL in pMMB208This study
 pMS56 epsL154exeL in pMMB208This study
 pMS57 exeL216epsL in pMMB208This study
 pMS59 exeL57epsL in pMMB208This study
 pMS60 epsL296exeL in pMMB208This study
 pMS73 epsL57exeL in pMMB208This study
 pMS74 exeL296epsL in pMMB208This study
 pMS77 epsE epsL in pMMB208This study
 pMS79 epsE exeL296epsL in pMMB208This study
 pMS92 epsL216exeL in pMMB208This study
 pMS96 exeE exeL154epsL in pMMB208This study
 pMS97 exeE exeL216epsL in pMMB208This study
 pMS98 epsE epsL154exeL in pMMB208This study
 pMS99 epsE epsL216exeL in pMMB208This study
 pMS100 epsE epsL296exeL in pMMB208This study
 pMS101 exeE exeL296epsL in pMMB208This study
 pRJ84.1 exeL in pMMB208This study
 pT7-5T7 RNA polymerase promoter φ10, Apr 40
 pWD600 etxA etxB (E. coli heat-labile enterotoxin A and B subunit genes), Tcr 5