Table 1.

E. coli strains and plasmids used in this study

Strain or plasmidGenotypeSource or reference
Strains
DH5αΔ(argF lac)U196 endA1 recA1 hsdR17 (rK mK +) supE44 thi-1 gyrA1 relA1 (F′ φ80 ΔlacZΔM15) 8
AA93Δfec aroB Δ(argF lac)U169 araD139 rspL150 relA1 deoC1 flbB5301 ptsF25 rbsR 15
BL21(DE3)F hsdS galT; phage T7 polymerase under lacUV5control 25
SU202 lexA71::Tn5 sulA211 sulA::lacZ Δ(lacIPOZYA)169 F′lacI q lacZΔM15::Tn9 5
Plasmids
pT7-7Phage T7 promoter; ori(ColE1) Apr 26
pHSG576pSC101 derivative; Cmr 27
pMLB1034 ori (ColE1) Apr 22
pET25b(+)Phage T7 promoter; C-terminal His-tag; ori (ColE1) Apr Novagen
pET19bPhage T7 promoter; N-terminal His-tag; ori (ColE1) Apr Novagen
pFecRHispET25b(+) fecR This study
pHisFecRpET19b fecR This study
pIS136pHSG576 fecI 15
pIS135pHSG576 fecI fecR 15
pIRHispHSG576 fecI fecRhis This study
pIHisRpHSG576 fecIhisR This study
pIS1034pMLB1034 fecA; fecA-lacZfusion 15
pSV66pHSG576 fecI fecR fecA 9
pAA70pT7-7fecR 29
pAA71pT7-7fecI 1
pIS711pT7-7fecA 10
pSP47pT7-7fecAΔ47–101 10
pDP804LexA1–87408-Jun zipper 5
pMS604LexA1–87WT-Fos zipper 5
pUS10LexA1–87WT-FecA1–79 a This study
pUS11LexA1–87408-FecR101–317 a This study
pUS12LexA1–87408-FecR151–317 a This study
pUS13LexA1–87408-FecR101–275 a This study
pSM173LexA1–87WT-FecI1–173 a This study
pSM133LexA1–87WT-FecI1–133 a This study
pSM4LexA1–87WT-FecI82–173 a This study
pSM85LexA1–87408-FecR1–85 a This study
pSM9LexA1–87408-FecR9–85 a This study
pSM19LexA1–87408-FecR19–85a This study
pSM38LexA1–87408-FecR1–38 a This study
pSM58LexA1–87408-FecR1–58 a This study
  • a Subscripts indicate the amino acid residues contained in the protein deletion derivatives.