Table 1.

Strains and plasmids

Strain or plasmidRelevant characteristic(s)Source or reference
E. coli
 DH5αrecA1 endA1 hsdR17(rKmK+) supE44 thi-1 gyrA relA1λ φ80d/lacZΔM15 (ΔlacZYA-argF)U169Laboratory stock
A. tumefaciens
 A136C58 heat cured of pTiC58Laboratory stock
 A348A136 containing pTiA6Laboratory stock
 A348ΔB8A348 containing a deletion in pTiA6 virB85
Saccharomyces cerevisiae
 EGY48ura3 his3 trp1LexAop-leu216
 AD842EGY48 containing pSH18-3411
Plasmids
 pEG202Cloning vector for the construction of LexA fusions16
 pJK202Derivative of pEG202 with a nuclear localization signalA. Peterson
 pJG4-5Cloning vector for the construction of activator fusions16
 pSH18-34A plasmid containingGal1-LexAop-lacZ, a reporter gene in yeast16
 pAD14830.25-kbEcoRI fragment containing virB7 obtained by PCR amplification in pEG202 (activator-VirB7 A11 fusion; bp 6261–6509 of Ward et al. [26])This study
 pAD15221.0-kb EcoRI fragment obtained by PCR amplification in pJK202 (LexA-VirB10 I47 fusion; bp 8111–9108)This study
 pAD15230.6-kb EcoRI fragment obtained by PCR amplification in pJK202 (LexA-VirB8 T60 fusion; bp 6562–7197)This study
 pAD16630.6-kb EcoRI-XhoI fragment of virB8S87L obtained by PCR amplification in pJK202 (LexA-VirB8S87L fusion at T60)This study
 pAD15260.9-kbEcoRI fragment obtained by PCR amplification in pJK202 (LexA-VirB9 T17 fusion; bp 7143–8019)This study
 pAD14470.25-kb EcoRI fragment containingvirB7 obtained by PCR amplification of pAD1399 in pJG4-5 (activator-VirB7 A11 fusion)11
 pAD14931.0-kb EcoRI fragment obtained by PCR amplification in pJG4-5 (activator-VirB10 I47 fusion; bp 8111–9108)This study
 pAD15160.6-kbEcoRI-XhoI fragment obtained by PCR amplification in pJG4-5 (activator-VirB8 T60 fusion; bp 6562–7197)This study
 pAD15170.9-kb EcoRI fragment obtained by PCR amplification in pJG4-5 (activator-VirB9 T17 fusion; bp 7143–8019)This study
 pAD15290.35-kb EcoRI fragment obtained by PCR amplification in pJK202 (LexA-VirB8 T60-T172 fusion; bp 6562–6900)This study
 pAD15300.33-kbEcoRI fragment obtained by PCR amplification in pJK202 (LexA-VirB8 P145-P237 fusion; bp 6817–7098)This study
 pAD15310.33-kb EcoRI fragment obtained by PCR amplification in pJK202 (LexA-VirB9 T17-H122 fusion; bp 7143–7460)This study
 pAD15330.45-kb EcoRI fragment obtained by PCR amplification in pJK202 (LexA-VirB9 D75-P220 fusion; bp 7317–7754)This study
 pAD14920.4-kbEcoRI fragment obtained by PCR amplification in pJK202 (LexA-VirB9 L173-293 fusion; bp 7611–8019)This study
 pAD15280.7-kb EcoRI-SalI fragment obtained by PCR amplification in pJK202 (LexA-VirB10 I47-V277 fusion; bp 8111–8803)This study
 pAD15340.65-kb EcoRI fragment obtained by PCR amplification in pJK202 (LexA-VirB10 R161-377 fusion; bp 8453–9108)This study
 pAD15981.0-kbEcoRI fragment of pAD1493 in pRsetB (His-VirB10 fusion)This study
 pAD15991.0-kb EcoRI fragment of pAD1493 in pGEX1λT (GST-VirB10 fusion)This study
 pAD16010.6-kb EcoRI fragment of pAD1523 in pGEX1λT (GST-VirB8 fusion)This study
 pAD16060.9-kbEcoRI fragment of pAD1517 in pGEX1λT (GST-VirB9 fusion)This study