Table 3.

Influence of inhibitors on the DNA polymerase activity from P. islandicum

InhibitorConcentrationaDNA polymerase activity (%)b
NEMc0100
140
230
50
Aphidicolin0 (0)100
50 (17)100
200 (68)70
500 (170)55
2,000 (680)21
ddGTP5 95
10 80
20 53
  • a The concentrations of the NEM inhibitor are millimolar, and the concentrations of the aphidicolin inhibitor are micromolar and, in parentheses, micrograms per milliliter. The concentrations of the ddGTP inhibitor are ratios of ddGTP/dGTP.

  • b The influence of different inhibitors was detected at standard assay conditions (pH 7.3; 0.5 mM MgCl2; 0 mM KCl; 75°C) using 0.2 U of purified DNA polymerase.

  • c A preincubation of the enzyme with NEM was performed at 90°C for 20 min.