Table 4.

β-Galactosidase activity by the bacterial two-hybrid Lex-based system in E. coliSU202 sulA::lacZa

Protein combinationPercentage of β-galactosidase activity ± SD
LexA1–87408-Jun zipper100 ± 22
LexA1–87WT-Fos zipper100 ± 18
LexA1–87408-Jun zipper and LexA1–87WT-Fos zipper7 ± 3
LexA1–87WT-FecA1–87 100 ± 6
LexA1–87408-FecR101–317 100 ± 4
LexA1–87WT-FecA1–79 and LexA1–87408-Jun zipper102 ± 6
LexA1–87408-FecR101–317 and LexA1–87WT-Fos zipper88 ± 3
LexA1–87WT-FecA1–79 and LexA1–87408-FecR101–317 9 ± 2
LexA1–87WT-FecA1–79 and LexA1–87408-FecR151–317 124 ± 25
LexA1–87WT-FecA1–79 and LexA1–87408-FecR101–275 107 ± 13
LexA1–87WT-FecI1–173 85 ± 17
LexA1–87408-FecR1–85 96 ± 14
LexA1–87408-Jun zipper and LexA1–87WT-FecI1–173 113 ± 19
LexA1–87WT-Fos zipper and LexA1–87408-FecR1–85 120 ± 12
LexA1–87WT-FecI1–173 and LexA1–87408-FecR1–85 14 ± 4
LexA1–87WT-FecI1–173 and LexA1–87408-FecR9–85 12 ± 4
LexA1–87WT-FecI1–173 and LexA1–87408-FecR19–85 102 ± 19
LexA1–87WT-FecI1–173 and LexA1–87408-FecR1–58 14 ± 5
LexA1–87WT-FecI1–173 and LexA1–87408-FecR1–38 107 ± 10
LexA1–87WT-FecI1–133 and LexA1–87408-FecR1–85 80 ± 21
LexA1–87WT-FecI82–173 and LexA1–87408-FecR1–85 111 ± 23
  • a The activity of the various protein combinations is shown as the relative percentage of β-galactosidase activity. An activity of 100% corresponds to approximately 250 Miller units. Percentage indicated is an average over three experiments; the standard deviation is shown.