Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaSource or reference
Strains
 NT1pTiC58-cured derivative of C58, contains pAtC58Our collection
 C58C1RSpTiC58-cured derivative of C58, Rifr Strr, contains pAtC58 37
Plasmids
 pTiC58Wild-type Ti plasmid, Noc+ Acc+Trai Our collection
 pTiC58ΔaccR accR deletion derivative of pTiC58, Noc+ AcccTrac 1
 pCMA1 traM::nptII mutant of pTiC58, Noc+ Acc+ TracKanr 21
 pJM749pTHB58traA::lacZ749 1
  • a Noc+, regulated catabolism of nopaline; Acc+, regulated catabolism of agrocinopines A and B; Accc, constitutive catabolism of agrocinopines A and B; Trai, inducible for conjugal transfer; Trac, constitutive for conjugal transfer; Rifr, rifampin resistant; Strr, streptomycin resistant; Kanr, kanamycin resistant.