Table 2.

Induction time influences development of conjugation competency

Induction medium supplementDonor titers and transfer frequencies following induction for:
15 h20 h
Donor density (CFU/cm2)Transfer frequencyaDonor density (CFU/cm2)Transfer frequencya
Agrocinopines1 × 106 <10−6 1 × 106 <10−6
2 × 107 <10−7 3 × 107 3 × 10−5
4 × 108 7 × 10−6 2 × 108 1 × 10−4
Agrocinopines plus AAI1 × 106 <10−6 3 × 106 1 × 10−4
2 × 107 2 × 10−5 4 × 107 3 × 10−4
3 × 108 4 × 10−5 3 × 108 1 × 10−4
  • a Expressed as the number of transconjugants recovered per input donor. The experiment was repeated once, yielding a similar patterns of results.