Table 1.

Genetic identificationa of biofilm-defective mutants of S. gordonii Challis

MutantAlleleLocus% of level of wild-type biofilm formationbGenetic functionPutative role in biofilmNucleotide sequence at Tn916junctioncAccession no.Reference
18F9 comD 61.4Histidine kinaseQuorum sensingTTAATTTTATTGTAA U80077 42
21C1PBP 2B gene9.0Peptidoglycan biosynthesisOsmoregulation?AAGATTTTTATACTA AF207585 This study
311E5LPBP 5 gene44.3Peptidoglycan biosynthesisOsmoregulation?ATTATTTTTTAAAGA AF207578 This study
11E5UATPase geneCation transportOsmoregulation?CTATATTTTTAAAGA AF207579 This study
411B4 glmM 55.5Peptidoglycan biosynthesisOsmoregulation?GTTATTTTTTTATTA AF207577 This study
515B3 bacA 12.6Peptidoglycan biosynthesisOsmoregulation?TACTTTTATACTTTA AF207587 This study
69F8 abpA 16.2α-Amylase bindingAdhesionCTCCTTTTTTATGTA AF035817 51
729E5 appC 24.9Oligopeptide transportNutrient sensing?AGTTTTTTTTATGTA AF207584 This study
84B3 mutT 17.1DNA replicationUnknownTTTATTATTAATTTA AF207581 This study
913A12 ytmP 14.6UnknownUnknownTTATTTTTTTTATTA AF207592 This study
108A11No match35.3UnknownUnknownGTATTTTTTACAAAA AF207586 This study
116C12No match28.2UnknownUnknownATAATTTTTTATTTA AF207591 This study
1214H6No match26.1UnknownUnknownGTTCTTTTTATTCAA AF207582 This study
138B8No match33.2UnknownUnknownTGATTTTTTTGAATA AF207583 This study
1412E11No match18.5UnknownUnknownCTATATTTTTAAAGA AF207576 This study
158G2No match21.6UnknownUnknownCGAATTTTAATTTTA AF207580 This study
1629F1No match43.3UnknownUnknownGTTCTTTTTAATGTA AF207590 This study
1718D10No match22.1UnknownUnknownGCTATTATTATACCA AF207588 This study
1811G1No match32.1UnknownUnknownTCGATTTTTTTATTA AF207589 This study
  • a Based on DNA homologies with DNA sequences in GenBank.

  • b Wild-type biofilm formation was considered to be 100%.

  • c Fifteen nucleotides upstream of the chromosomal DNA of the Tn916 insertion.