Table 1.

Genetic identificationa of biofilm-defective mutants of S. gordonii Challis

MutantAlleleLocus% of level of wild-type biofilm formationbGenetic functionPutative role in biofilmNucleotide sequence at Tn916junctioncAccession no.Reference
18F9comD61.4Histidine kinaseQuorum sensingTTAATTTTATTGTAAU8007742
21C1PBP 2B gene9.0Peptidoglycan biosynthesisOsmoregulation?AAGATTTTTATACTAAF207585This study
311E5LPBP 5 gene44.3Peptidoglycan biosynthesisOsmoregulation?ATTATTTTTTAAAGAAF207578This study
11E5UATPase geneCation transportOsmoregulation?CTATATTTTTAAAGAAF207579This study
411B4glmM55.5Peptidoglycan biosynthesisOsmoregulation?GTTATTTTTTTATTAAF207577This study
515B3bacA12.6Peptidoglycan biosynthesisOsmoregulation?TACTTTTATACTTTAAF207587This study
69F8abpA16.2α-Amylase bindingAdhesionCTCCTTTTTTATGTAAF03581751
729E5appC24.9Oligopeptide transportNutrient sensing?AGTTTTTTTTATGTAAF207584This study
84B3mutT17.1DNA replicationUnknownTTTATTATTAATTTAAF207581This study
913A12ytmP14.6UnknownUnknownTTATTTTTTTTATTAAF207592This study
108A11No match35.3UnknownUnknownGTATTTTTTACAAAAAF207586This study
116C12No match28.2UnknownUnknownATAATTTTTTATTTAAF207591This study
1214H6No match26.1UnknownUnknownGTTCTTTTTATTCAAAF207582This study
138B8No match33.2UnknownUnknownTGATTTTTTTGAATAAF207583This study
1412E11No match18.5UnknownUnknownCTATATTTTTAAAGAAF207576This study
158G2No match21.6UnknownUnknownCGAATTTTAATTTTAAF207580This study
1629F1No match43.3UnknownUnknownGTTCTTTTTAATGTAAF207590This study
1718D10No match22.1UnknownUnknownGCTATTATTATACCAAF207588This study
1811G1No match32.1UnknownUnknownTCGATTTTTTTATTAAF207589This study
  • a Based on DNA homologies with DNA sequences in GenBank.

  • b Wild-type biofilm formation was considered to be 100%.

  • c Fifteen nucleotides upstream of the chromosomal DNA of the Tn916 insertion.