Table 1.

B. subtilis strains used in this study

StrainRelevant genotypeSource or reference
UOT1285 trpC2 lys 1 aprEΔ3 nprE18 nprR2 Laboratory stock
SK6 trpC2 lys 1 aprEΔ3 nprE18 nprR2 shaA::neo This study
RIK10 trpC2 lys 1 aprEΔ3 nprE18 nprR2 amyE::spo0A (PS)-bgaB cat 38
RIK51 trpC2 lys 1 aprEΔ3 nprE18 nprR2 amyE::spoVG-bgaB cat 1
RIK50 trpC2 lys 1 aprEΔ3 nprE18 nprR2 amyE::spo0H-bgaB cat 38
SK610SK6amyE::spo0A (PS)-bgaB cat SK6 DNA→RIK10a
SK651SK6amyE::spoVG-bgaB cat SK6 DNA→RIK51a
SK650SK6amyE::spo0H-bgaB cat SK6 DNA→RIK50a
RIK62 trpC2 lys 1 aprEΔ3 nprE18 nprR2 sof-1 spo0FΔS amyE::spo0A (PS)-bgaB cat This study
SK662 trpC2 lys 1 aprEΔ3 nprE18 nprR2 sof-1 spo0FΔS amyE::spo0A(PS)-bgaB cat shaA::neo SK6 DNA→RIK62a
  • a RIK10, RIK51, RIK50, and RIK62 were each transformed with chromosomal DNA from strain SK6 to construct SK610, SK651, SK650, and SK662, respectively.