Table 1.

Comparisons of enzymatic properties among CL190, E. coli, and pepper DXP synthases

DXP synthase sourceKmfor:Vmax (U/mg of protein)Optimum temp (°C)Optimum pHaActivation energy (kJ/mol)Divalent cationsMolecular mass (kDa) by:Multimeric form
Pyruvate (μM)d-Glyceraldehyde 3-phosphate (μM)d-Glyceraldehyde (mM)SDS-PAGEGel filtration chromatography
CL190651203537042–449.099Mg2+, Mn2+ 70130Dimer
E. coli 962403830042–447.5–8.063Mg2+, Mn2+ 69120Dimer
Pepperb 500750
  • a Assay solutions consisted of 100 mM Tris-HCl at pH 7.0 to 9.5.

  • b From Bouvier et al. (1).