Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant genotypeConstructiona or source
Bacillus strains
 PY22 trpC2 P. Youngman
 BSA46 trpC2 SPβctc::lacZ 3
 XS352 trpC2 aph3′5"/sigB rsbST 18
 BSA419 trpC2 Pspac ::rsbTSPβ ctc::lacZ 17
 BSJ13 trpC2 Pspac ::rsbT Pxyl ::obg SPβctc::lacZ 17
 BSJ38 trpC2 Pspac ::rsbT rsbX::spec SPβctc::lacZ BSA625 → BSA419
 BSJ39 trpC2 aph3′5"/sigB ΔrsbST XS352 → PY22
 BSJ40 trpC2 aph3′5"/sigB ΔrsbST Pspac ::rsbT SPβctc::lacZ pHV501T → BSJ39
 BSJ41 trpC2 aph3′5"/sigB ΔrsbST Pspac ::rsbT SPβctc::lacZ BSA46 → BSJ40
 BSJ42 trpC2 aph3′5"/sigB rsbRΔ5 Pspac ::rsbT SPβctc::lacZ pJM49 → BSA419
Plasmids
 pHV501T AprEmrPspac ::rsbT 17
 pRS11Apr Kanr PA ::rsbR rsbS 18
 pJM49AprKanr PA ::rsbRΔ5 rsbS This study
 pML7/X::specAprCmr Spr PBrsbVW sigB rsbX::spec 4
  • a Transformations were performed as described by Yasbin et al. (29).