Table 1.

Plasmidsa

PlasmidConstruction
pOS530 pra gene promoter (a fragment from nt 1766 to 1904 in AJ005260) from pSAM2B2 cloned after PCR amplification as theBamHI-EcoRI fragment in the promoter probe vector pIJ487/Hygro
pOS556 BamHI(154)-BamHI(1746) fragment of pSAM2B3 containing the korSA gene cloned in the integrative vector pTO1* (22);orf131 present in the fragment was inactivated by deletion of the BglII(297)-BsrGI(955) fragment; contains the tsr gene conferring resistance to thiostrepton
pOS699pSAM2B3 derivative with the korSAgene inactivated by deletion of the NcoI-NcoI fragment; the NcoI site was modified by Klenow polymerase, introducing a frameshift
pOS702 korSA gene promoter (a fragment from nt 884 to 995 in AJ005260) from pSAM2B3cloned after PCR amplification as theBamHI-HindIII fragment in the promoter probe vector pIJ487/Hygro
pIJ487/HygropIJ487 (32) derivative in which the hyg gene (33) was cloned in the EcoRV site; Hygr Tsrs
  • a All plasmids were constructed in this work.