Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or descriptionReference or Source
M. catarrhalis
 035EWild-type disease isolate, attaches to Chang cells, serum resistant2
 035E.1uspA1 mutant of 035E, attachment deficient, serum resistant2
 035E.2uspA2 mutant of 035E, attaches to Chang cells, serum sensitive2
 035E.12uspA1 uspA2 mutant of 035E, attachment deficient, serum sensitive2
 012EWild-type disease isolate, attaches to Chang cells, serum resistant1
 012E.1uspA1mutant of 012E, attachment deficient, serum resistantThis study
 012E.2uspA2 mutant of 012E, attaches to Chang cells, serum sensitiveThis study
 012E.12uspA1 uspA2 mutant of 012E, attachment deficient, serum sensitiveThis study
 TTA37Wild-type disease isolate, attaches to Chang cells, serum sensitiveSteven Berk
 TTA37.1uspA1 mutant of TTA37, attaches to Chang cells, serum sensitiveThis study
 TTA37.2uspA2Hmutant of TTA37, attachment deficient, serum sensitiveThis study
 TTA37.12uspA1 uspA2H mutant of TTA37, attachment deficient, serum sensitiveThis study
 046EWild-type disease isolate, attaches to Chang cells, serum resistantJohn Nelson
 046E.1uspA1 mutant of 046E, attaches to Chang cells, serum resistantThis study
 046E.2uspA2H mutant of 046E, attaches to Chang cells, serum sensitiveThis study
 046E.12uspA1 uspA2H mutant of 046E, attachment deficient, serum sensitiveThis study
 TTA24Wild-type disease isolate10
 P44Wild-type disease isolate25
 ATCC 25240Wild-type disease isolateAmerican Type Culture Collection
 E22Wild-type disease isolate5
 V1166Wild-type isolate from nasopharynx of a healthy childF. Henderson
 V1171Wild-type isolate from nasopharynx of a healthy child10
E. coliDH5αHost strain for cloning experiments37
H. influenzae DB117Host strain for cloning experiments39
Plasmids
 pBS KS(+)Cloning vector, AmprStratagene
 pUSPA1KANpBS containing a truncated uspA1 gene from M. catarrhalis strain 035E into which a kanamycin resistance cartridge was inserted3
 pSPECrSource of the spectinomycin resistance cartridge42
 pELU2P44SPECpBS containing an incomplete uspA2 gene from M. catarrhalis P44 into which a spectinomycin resistance cartridge was insertedThis study
 pACYC184Cloning vectorNew England Biolabs
 pELU112pACYC184 containing the M. catarrhalis 012EuspA1 geneThis study
 pELU212pACYC184 containing the M. catarrhalis 012E uspA2geneThis study
 pELU135pACYC184 containing the M. catarrhalis 035E uspA1 geneThis study
 pELU235pACYC184 containing the M. catarrhalis 035EuspA2 geneThis study
 pELU146pACYC184 containing the M. catarrhalis 046E uspA1geneThis study
 pELU246pACYC184 containing the M. catarrhalis 046E uspA2H geneThis study
 pELU137pACYC184 containing the M. catarrhalisTTA37 uspA1 geneThis study
 pELU237pACYC184 containing the M. catarrhalis TTA37 uspA2HgeneThis study
 pELU171pACYC184 containing the M. catarrhalis V1171 uspA1 geneThis study
 pELU271pACYC184 containing the M. catarrhalisV1171 uspA2 geneThis study
 pELU266pACYC184 containing the M. catarrhalis V1166 uspA2HgeneThis study