Table 4.

Adherence of recombinant H. influenzae cells to Chang conjunctival epithelial cells in vitro and detection of recombinant proteins on the bacterial cell surface

Recombinant plasmidM. catarrhalisproteinaAdherencebDetection of protein on cell surfacec
pACYC184 (vector)0.2 ± 0.1918 ± 276
pELU112012E-UspA135.1 ± 8.713,870 ± 2,132
pELU212012E-UspA20.4 ± 0.25,557 ± 1,546
pELU135035E-UspA117.4 ± 7.35,050 ± 1,184
pELU235035E-UspA20.2 ± 0.17,175 ± 3,201
pELU171V1171-UspA112.3 ± 7.26,425 ± 1,049
pELU271V1171-UspA20.4 ± 0.24,111 ± 1,333
pELU137TTA37-UspA10.9 ± 0.41,286 ± 206
pELU237TTA37-UspA2H16.9 ± 6.311,630 ± 1,351
pELU146046E-UspA10.8 ± 0.2703 ± 221
pELU246046E-UspA2H52.6 ± 15.1728,980 ± 1,703
pELU266V1166-UspA2H62.3 ± 16.65,632 ± 199
  • a The M. catarrhalis protein expressed by the H. influenzae DB117 clone containing this recombinant plasmid.

  • b Adherence of M. catarrhalisorganisms to Chang cells is expressed as the mean (± standard deviation) percentage of the inoculum which adhered to the monolayers.

  • c Counts per minute (mean ± standard deviation) of radioiodinated goat anti-mouse immunoglobulin bound to MAb 17C7 on the surface of recombinant cells. The indirect antibody-accessibility assay was performed as described previously (2).