Table 1.

Purification of CFLE ofB. cereusa

ProcedureTotal protein (mg)Total activity (U)Sp act (U/mg of protein)Yield (%)Purification (fold)
Germination exudate39.31,13328.81001
SP-Sephadex C25 column0.535421,022.647.835.5
Hydroxyapatite0.113393,081.829.9107.0
Superose 120.0311695,451.614.9189.3
  • a B. cereus spores (5 g [packed weight]) were germinated as described in Materials and Methods. After centrifugation (8,000 × g for 10 min at 4°C), the supernatant was used as a germination exudate.