Table 2.

Amplification primers for probes specific forSfiI fragments of each chromosome

Probe (accession no.)SequenceChromosome (SfiI fragment)Annealing temp (°C)
ACT1(X16377)GGA CTT GTG TTG TTA TCT GG1 (L)50
AGA GAA AAT ACC CGT GAC G
HGT1 (Y16834)CAG TCT TCA TAC ATA CCC CGG1 (S)50
CAG GAT TTT CAC GAT CAC C
SAS2 (1946R)a TTC TGA AGG ATC AAT TGC C2 (U)50
AAA CCG ATT TAT CGT CAA G
GCN1 (S14D5)a CGC TTG TGG AAG TAC TTG GG2 (A)58
GCT TGT CGT CAG ACT CTT GTA AGG
ILV2(265169F08.y1.seq)a ATA GCA GTA TTG GCA GCA GC3 (O)55
ACC ATC ACA TGC ATT GAG TC
YER132 (p79F)a GCC ATC ATC GAC AGT ATC C3 (P)50
CCC AAT AAG TAT GTT TGC C
YJR61 (p356R)a TTC CGC TAC GGT ATT CTC4 (H)50
AAC CAG ACG TTA GTT GTC G
HIS4 (AJ003115)CCA GAC AGG AGA ATC CAA CG4 (BB)55
CCT AGA GCT TGG TGA ACC AGC
HEX1 (L26488)GTG AAG CTG CTT TAT GGT CGG A5 (I)58
AGT ATC ATC GAG ACT CGC GTG ACT
GCD11 (1997R)a TAC CAC CTT ATG GAA GTG GAC5 (M)60
GGT TCT ACT CCA ACT GGT GC
TSM1 (G4C4R)a CTT CCT GAC ATT GCC TGA AG6 (C)55
CCA CCA GAT ATG AGT GAC CC
ALS1 (L25902)AAC ATG TAC TGT GAA CGA CGC6 (O)60
AAT GGA CGA TAA CCA GCA GG
p321 (gel39821)a TGC AAC ATT GAT GGC AGC7 (C)60
TGC AGT TGG AGT CTC AGT CG
p143 (gel40106)a GCG CGC TTC CAT TAC TAG G7 (G)60
ACG TTC AGC AGA GAA CCA GG
INO1 (L22737)ATC TAA TGT GGC TAC GGT GCR (D)55
CCT TAG TGA CAG AAT TGG ACG
ADE1 (L12463)CCA CCG ACA GAA TTT CTG CR (M, S)55
GGC TAC ACC ATC TTT ACC AGC