Table 1.

Bacterial strains and plasmids used in this studya

Bacterial strain or plasmidRelevant genotype or descriptionSource or reference
Bacterial strains
B. subtilis
  168 trpC2 C. Anagnostopoulos
  XM15 trpC2 amyE::dra-lacZ 14
  XM25 trpC2 amyE::dra-lacZ deoR::erm 14
  XM251 trpC2 amyE::dra-lacZ deoR::erm pXM1000Transformation of XM25 by pXM1000, Plr
E. coli
  JOY999TG1(pQE-30)This work
  JOY1000TG1(pJOY1000)This work
  TG1Wild type;lacI q Laboratory stock
Plasmids
 pEB112Apr (E. coli) Plr(B. subtilis); multiple copy shuttle vector containing the pBR322 rep. origin for replication in E. coli and pC194 rep. origin for replication in B. subtilis 6
 pJOY1000 BamHI-HindIII PCR fragment containing deoR generated by primers S3 and S4, ligated to pQE-30 digested with BamHI andHindIIIThis work
 pXM1000 PstI-HindIII PCR fragment containing deoR generated by primers S4 and S5, ligated to pEB112 digested with PstI and HindIIIThis work
 pQE-30Apr, has a promoter and operator element consisting of the E. coli phage T5 promoter and twolac operator sequences, used for overexpressingdeoR Qiagen
 pHH1002 12
  • a Apr, ampicillin resistance; Plr, phleomycin resistance; rep., replication.