Table 1.

Strains and plasmids

Bacterial strain or plasmidRelevant featuresReference or source
Strains
H. pylori
  G27Clinical isolate 43
  G46Clinical isolate 43
  G50Clinical isolate 43
  G25Clinical isolate 43
  CCUG17874Clinical isolate 43
  G27[flgR]G27 with a kanamycin resistance cassette replacing the flgR (HP703) gene 35
  G27/HP244::kmG27 with a kanamycin resistance cassette partially replacing the ORF for HP244This study
  G27/HP165::kmG27 with a kanamycin resistance cassette replacing the ORF for HP165This study
  G27/HP1364::kmG27 with a kanamycin resistance cassette replacing the ORF for HP1364This study
  G27/HP1365::kmG27 with a kanamycin resistance cassette inserted into the ORF for HP1365This study
E. coli
  DH5αStrain used for high-efficiency transformationGibco
  M15Strain used for cloning and overproducing His6 tag fusionsQiagen
Plasmids
 pBluescript SKCloning vectorStratagene
 pSL1180Cloning vectorPharmacia
 pGEX-3XGST gene fusion and expression vectorPharmacia
 pQE-30Expression vector for N-terminal His6 tag cloningQiagen
 pREP4Derivative of pACYC overexpressing LacIQiagen
 pILL600Plasmid containing the kanamycin resistance cassette from C. coli 21
 pSL-244::kmpSL1180 containing the kanamycin resistance cassette flanked by EcoRI/BamHI andBamHI/PstI fragments of 541 bp and 458 bp derived from the ORFs for HP245 and HP243, respectivelyThis study
 pSL-165::kmpSL1180 containing the kanamycin resistance cassette flanked by EcoRI/BamHI andBamHI/PstI fragments of 463 bp and 635 bp derived from the ORFs for HP166 and HP163, respectivelyThis study
 pSL-1364::kmpSL1180 containing the kanamycin resistance cassette flanked by EcoRI/BamHI andBamHI/PstI fragments of 405 bp and 655 bp derived from the ORFs for HP1365 and HP1363, respectivelyThis study
 pSL-166::kmpSL1180 containing the kanamycin resistance cassette flanked by EcoRI/BamHI andBamHI/PstI fragments of 330 bp and 488 bp derived from the ORFs for HP166 and HP164, respectivelyThis study
 pSL-1365::kmpSL1180 containing the kanamycin resistance cassette flanked by EcoRI/BamHI andBamHI/PstI fragments of 304 bp and 484 bp derived from the ORFs for HP1365 and HP1364, respectivelyThis study
 pSL-1043::kmpSL1180 containing the kanamycin resistance cassette flanked by EcoRI/BamHI andBamHI/PstI fragments of 505 bp and 586 bp derived from noncoding DNAThis study
 pSL-1021::kmpSL1180 containing the kanamycin resistance cassette flanked by EcoRI/BamHI andBamHI/PstI fragments of 758 bp and 632 bp derived from the ORFs for HP1020 and HP1022, respectivelyThis study
 pGEX-244pGEX-3X expressing the transmitter domain of HP244 (aa 149 to 381) fused to GSTThis study
 pGEX-165pGEX-3X expressing the transmitter domain of HP165 (aa 167 to 414) fused to GSTThis study
 pGEX-165(C13)pGEX-3X expressing the transmitter domain of HP165 from H. pylori G46 fused to GSTThis study
 pGEX-165(C9)pGEX-3X expressing the transmitter domain of HP165 from H. pylori CCUG17874 with a frameshift introduced by site-directed mutagenesisThis study
 pGEX-1364pGEX-3X expressing the transmitter domain of HP1364 (aa 183 to 397) fused to GSTThis study
 pQE-166pQE30 expressing the His6-tagged response regulator HP166This study
 pQE-1365pQE30 expressing the His6-tagged response regulator HP1365This study
 pQE-703pQE30 expressing the His6-tagged response regulator HP703This study
 pQE-1043pQE30 expressing the His6-tagged response regulator HP1043This study
 pQE-1021pQE30 expressing the His6-tagged response regulator HP1021This study
 pQE-1021RpQE30 expressing the His6-tagged receiver domain (aa 3 to 118) of response regulator HP1021This study
 pSK-165(C11)pBluescript SK carrying a BamHI/EcoRI fragment encoding the transmitter domain of HP165 from H. pylori CCUG17874This study