Table 3.

Predicted values of phase transitions in L. monocytogenes membrane vesicles as measured by solute gradient-mediated enzymatic activity and FTIR spectroscopya

ConditionbTm(FTIR) (°C)Tm(enz) (°C)
600 mOsm KCl9.79.5
18.417.4
600 mOsm sucrose9.710.4
18.418.3
  • a Abbreviations:Tm(FTIR) , phase transition temperature as predicted by log percent maximum wave number as measured by FTIR spectroscopy; Tm(enz) , phase transition temperature as predicted by breaks in Arrhenius activation plots of enzymatic activity. Values were obtained by extrapolation of the curves in Fig. 3B and 4B.

  • b All systems were energized by addition of ascorbate-PMS.