Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaReference or source
E. coli
 Novablue endA hsdR17(rK mK +) supE thi recA gyrA relA lac[F′ proA + B + lacI q ZΔM15::Tn10(Tcr)]Novagen
 SM10-λpir thi thr leu supE tonA lacY recA::RP4-2-Tc::Mu Kmrλ::pir 50
V. fischeri
 ES114Wild type 4
 ESR1ES114, Rfr 34
 MJ-1Wild type 57
 MJR1MJ-1, Rfr This study
 MJ-100MJ-1, Nxr 18
 MJ-208MJ-100, ΔluxR 42
 MJ-211MJ-100, ΔluxI (nonpolar) 42
 MJ-215MJ-211, ainS 42
 MJ-216MJ-100,ainS::neo 33
 MJ-10XMJR1, qsrP::p300-10XMThis study
 ES-10XESR1,qsrP::p300-10XEThis study
 MJ-ribBXMJR1, ribB::p300-12XMThis study
Plasmids
 pT7BlueCloning vector; Apr Novagen
 pGP704R6K ori RP4mob Apr 50
 pSMC300pGP704; Δbla Cmr This study
 p300-10XEpSMC300; qsrP nt 119–269 from ESR1This study
 p300-10XMpSMC300; qsrP nt 119–269 from MJ-100This study
 p300-12XMpSMC300;ribB nt 194–450 from MJ-100This study
  • a nt, nucleotides.