Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaReference or source
E. coli
 NovablueendA hsdR17(rKmK+) supE thi recA gyrA relA lac[F′ proA+B+lacIqZΔM15::Tn10(Tcr)]Novagen
 SM10-λpirthi thr leu supE tonA lacY recA::RP4-2-Tc::Mu Kmrλ::pir50
V. fischeri
 ES114Wild type4
 ESR1ES114, Rfr34
 MJ-1Wild type57
 MJR1MJ-1, RfrThis study
 MJ-100MJ-1, Nxr18
 MJ-208MJ-100, ΔluxR42
 MJ-211MJ-100, ΔluxI (nonpolar)42
 MJ-215MJ-211, ainS42
 MJ-216MJ-100,ainS::neo33
 MJ-10XMJR1, qsrP::p300-10XMThis study
 ES-10XESR1,qsrP::p300-10XEThis study
 MJ-ribBXMJR1, ribB::p300-12XMThis study
Plasmids
 pT7BlueCloning vector; AprNovagen
 pGP704R6K ori RP4mob Apr50
 pSMC300pGP704; Δbla CmrThis study
 p300-10XEpSMC300; qsrP nt 119–269 from ESR1This study
 p300-10XMpSMC300; qsrP nt 119–269 from MJ-100This study
 p300-12XMpSMC300;ribB nt 194–450 from MJ-100This study
  • a nt, nucleotides.