Table 2.

MLVA primers used to DNA type B. anthracis

Marker locusPrimersPrimer sequenceDye labelaTm (°C)
vrrA vrrA-f1-fam CAC AAC TAC CAC CGA TGG CAC A Fam71.0
vrrA-r1 GCG CGT TTC GTT TGA TTC ATA C None69.7
vrrB 1 vrrB 1-f1-fam ATA GGT GGT TTT CCG CAA GTT ATT C Fam70.0
vrrB 1-r1 GAT GAG TTT GAT AAA GAA TAG CCT GTG None69.0
vrrB 2 vrrB 2-f1-fam CAC AGG CTA TTC TTT ATC AAA CTC ATC Fam72.0
vrrB 2-r1 CCC AAG GTG AAG ATT GTT GTT GA None68.8
vrrC 1 vrrC 1-f1 GAA GCA AGA AAG TGA TGT AGT GGA C None66.8
vrrC 1-r1-fam CAT TTC CTC AAG TGC TAC AGG TTC Fam67.5
vrrC 2 VrrC 2-f1-hex CCA GAA GAA GTG GAA CCT GTA GCA C Hex70.9
vrrC 2-r1 GTC TTT CCA TTA ATC GCG CTC TAT C None70.6
CG3CG3-f1-ned TGT CGT TTT ACT TCT CTC TCC AAT AC Ned66.2
CG3-r1 AGT CAT TGT TCT GTA TAA AGG GCA T None66.2
pXO1-aatpXO1-AAT-f3-fam CAA TTT ATT AAC GAT CAG ATT AAG TTC A Fam66.3
pXO1-AAT-r3 TCT AGA ATT AGT TGC TTC ATA ATG GC None66.7
pXO2-atpXO2-AT-f1-hex TCA TCC TCT TTT AAG TCT TGG GT Hex64.4
pXO2-AT-r1 GTG TGA TGA ACT CCG ACG ACA None65.8
  • a PE Biosystem's 5′ fluorescent phosphoramidite dyes: 6-Fam, Hex, and Ned.