Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant genotype or phenotypeReference
P. aeruginosa strains
 PAO1Wild type6
 PDO100rhlI::Tn501-2; derived from strain PAO12
 PAO-R1lasR::Tet; derived from strain PAO15
 PAO-JP2lasI::TetrhlI::Tn501-2; derived from strain PDO10018
Plasmids
 pLPRIrhlI′-lacZ transcriptional fusion26
 pPCS1001lasR′-lacZtranscriptional fusion20
 pPCS1002rhlR′-lacZ transcriptional fusion20
 pPCS223lasI′-lacZtranscriptional fusion26
 pLP170lacZ transcriptional fusion vector23
 pTS400lasB′-lacZtranslational fusion15
 pECP39trcp-ΔlasR; encodes autoinducer-independent form of LasR22