Table 1.

Bacteria and plasmids used in this study

Strain or plasmidCharacteristic(s)Source or reference
E. coli
 DH5αF recA1 endA1 hsdR17(rK mK +) supE44 gyr96 relA1Δ(lacZYA-argF)U169 φ80dlacZΔM15deoR thi Bethesda Research Laboratories
 S17-1Tpr Smr hsdR pro recARP4-2-Tc::Mu-Km::Tn7 in chromosome 38
R. sulfidophilum
 W4Wild type 20
 RESA1 regAΔPinAI-PinAI::Kmr 29
R. capsulatus ATCC 11166Wild type 35
Plasmids
 pUC119Apr, multiple cloning sites inlacZ 42
 pUFS101pUC118 with 10-kb EcoRI insert encoding R. sulfidophilum pufoperon 30
 pUFS0012,580-bpEcoRI-BamHI fragment containing pufQ,pufB, pufA, and part of pufL from pUFS101 cloned into EcoRI-BamHI-cut pUC119This study
 pCF1010Transcriptional fusion vector containing unique PstI, NotI, NsiI,AvrII, StuI, BspMII, andXbaI restriction sites between the 2.0-kb Ω Smr/Spr and the 5.1-kb lacZYA′, IncQ/IncP4 28
 pPS0012.4-kbHincII-XbaI fragment from pUFS001 cloned intoStuI-XbaI-cut pCF1010This study
 pPS0022.1-kb NruI-XbaI fragment from pUFS001 cloned into StuI-XbaI-cut pCF1010This study
 pPS0031.9-kb SmaI-XbaI fragment from pUFS001 cloned into StuI-XbaI-cut pCF1010This study
 pPS0041.5-kbPstI-XbaI fragment from pUFS001 cloned intoPstI-XbaI-cut pCF1010This study
 pPS0051.4-kb BalI-XbaI fragment from pUFS001 cloned into StuI-XbaI-cut pCF1010This study
 pPS1001.0-kb HincII-BalI fragment from pUFS001 cloned into StuI-cut pCF1010This study
 pPS200656-bp PCR product from pUFS001 template cloned intoStuI-XbaI-cut pCF1010This study
 pPS300374-bp PCR product from pUFS001 template cloned intoStuI-XbaI-cut pCF1010This study