Table 2.

Incorporation of d-[14C]alanine into permeabilized cells and membranes from L. casei 102S (dltD+ ) and itsdltD::erm mutant

GenotypeaIncorporation ofd-[14C]alanine (cpm)b
Permeabilized cellsMembranes
dltD+ 960 ± 201,660 ± 20
dltD + (heat treated)15 ± 440 ± 6
dltD::erm 55 ± 6890 ± 20
dltD + + (pNZ123/dlt)2,540 ± 201,790 ± 20
dltD::erm + (pNZ123/dlt)2,680 ± 201,840 ± 20
  • a dltD+ is the genotype ofL. casei 102S (wild type).

  • b Cells (260 μg [wet weight]) were permeabilized with 0.6% toluene and incubated with ATP andd-[14C]alanine according to thed-alanine incorporation assay described in Materials and Methods. Membranes (120 μg of protein) were incubated with ATP,d-[14C]alanine, and recombinant Dcl and Dcp from L. rhamnosus according to thed-alanine incorporation assay.