Table 5.

Effect of mutations in UME6, SIN3, and RPD3 on the starvation response of CAR1and CAR2 genes

StrainGenotypeMediumaExpression of:
CAR1cCAR2-lacZb
BY4709ura3M.ammonia7176
−Nd601,184
BY4709Iura3 ume6::kanMX4M.ammonia661,644
−N761,743
BY4709IIura3 sin3::kanMX4M.ammonia38900
−N631,165
BY4709IIIura3 rpd3::kanMX4M.ammonia34653
−N651,045
  • a When no plasmid was present in the strains, 25 μg of uracil was added.

  • b β-Galactosidase activity was measured in extracts from different strains transformed with plasmid pFV118 (CAR2-lacZ). The β-galactosidase specific activity is expressed in nanomoles ofo-nitrophenyl-β-d-galactopyranoside hydrolyzed per minute per milligram of protein.

  • c Arginase-specific activity is expressed in micromoles of urea produced per hour per milligram of protein. The standard error was 10 to 15%.

  • d −N, medium with no nitrogen source (see Materials and Methods).