Table 2.

Identification of CARGRIII gene as RPD3

ExptStrainPlasmidGenotypeArginase specific activity on M.ammoniaa
202451cura3 cargRIII50*
312T7cIIIura3 rpd3::kanMX433*
412T7cIIura3 sin3::kanMX449*
502451c × 12T7cIIIura3 cargRIII × ura3 rpd3::kanMX430*
602451c × 12T7cIIura3 cargRIII × ura3 sin3::kanMX412*
702451cpFV36cura3 cargRIII13
802451cpFV33bura3 cargRIII38
  • a Arginase specific activity was measured at 30°C after growth of the different strains on M.ammonia–25 μg of uracil per ml where indicated (∗). The specific activity is expressed in micromoles of urea produced per hour per milligram of protein. The values are the means of two independent assays, and the standard error was 10 to 15%.

  • b Corresponds to pRS416 CEN6 ARS4 URA3 SIN3.

  • c Corresponds to pRS416 CEN6 ARS4 URA3 RPD3.