Table 1.

Description of fluxes and metabolites constituting the flux model of C. glutamicum

FluxConversion of metabolitesaEnzyme reaction(s)b
v1 Glucose-6-P↔fructose-6-PGlucose-6-phosphate isomerase (EC
v2 Fructose-6-P↔2 glyceraldehyde-3-P'sPhosphofructokinase (EC, fructose-1,6-bisphosphate aldolase (EC, triose phosphate isomerase (EC
v3 Glyceraldehyde-3-P↔PEP/pyruvateGlyceraldehyde-3-phosphate dehydrogenase (EC, 3-phosphoglycerate kinase (EC, phosphoglycerate mutase (EC, enolase (EC
v4 Glucose-6-P↔pentose-5-P + CO2 Glucose-6-phosphate dehydrogenase (EC, lactonase (EC, 6-phosphogluconate dehydrogenase (EC1.1.1.44)
v5 2 Pentose-5-P's↔sedoheptulose-7-P + glyceraldehyde-3-PTransketolase (EC
v6 Sedoheptulose-7-P + glyceraldehyde-3-P↔fructose-6-P + erythrose-4-PTransaldolase (EC
v7 Erythrose-4-P + pentose-5-P↔fructose-6-P + glyceraldehyde-3-PTransketolase (EC2.2.1.1)
v8 PEP/pyruvate↔AcCoA + CO2 Pyruvate dehydrogenase complex (EC; EC2.3.1.12; EC
v9 AcCoA + malate/oxaloacetate↔isocitrateCitrate synthase (EC, aconitase (EC
v10 Isocitrate↔oxoglutarate + CO2 Isocitrate dehydrogenase (EC
v11 Oxoglutarate↔succinate + CO2 2-Oxoglutarate dehydrogenase (EC; EC2.3.1.61; EC, succinyl CoA synthetase (EC
v12 Succinate↔malate/oxaloacetateSuccinate dehydrogenase (EC, fumarase (EC
v13 Isocitrate + AcCoA↔succinate + malate/oxaloacetateIsocitrate lyase (EC, malate synthase (EC, succinate dehydrogenase (EC, fumarase (EC4.2.1.2)
v14 PEP/pyruvate + CO2↔malate/oxaloacetatePEP carboxylase (EC, PEP carboxykinase (EC, pyruvate carboxylase (EC, oxaloacetate decarboxylase (EC, malic enzyme (EC1.1.1.40)
  • a Pentose-5-phosphate combines ribose-5-phosphate, ribulose-5-phosphate, and xylulose-5-phosphate. P, phosphate; AcCoA, acetyl-CoA.

  • b Treating PEP and pyruvate, malate and oxaloacetate, and ribose-5-phosphate, ribulose-5-phosphate, and xylulose-5-phosphate as single pools precludes the assignment of fluxes for enzyme reactions interconverting these metabolites (pyruvate kinase [EC] and the PEP:glucose-phosphotransferase system [EC2.1.1.69; EC] for PEP/pyruvate, malate dehydrogenase [EC1.1.1.37] for malate/oxaloacetate, and ribose-5-phosphate isomerase [EC] and ribulose-5-phosphate epimerase [EC] for pentose-5-phosphates).