Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant genotype and/or phenotypeaSource or reference
V. cholerae
 O395Wild type (classical Ogawa); Smr 36
 C6709Wild type (El Tor Inaba); Smr 5
 JB29O395lacZ::toxT-gusA; Smr This study
 JB62JB29eno::TnphoA; SmrKmr This study
 JB65JB29rhlB::TnphoA; SmrKmr This study
 JB66JB29cadB::TnphoA; SmrKmr This study
 JB69JB29pepA::TnphoA; SmrKmr This study
 JB70JB29yhdA::TnphoA; SmrKmr This study
 JB73JB29argH::TnphoA; SmrKmr This study
 JB98JB29pepA::pJB10; SmrApr This study
 JB104YM2-34pepA::pJB10; SmrApr This study
 JB115JB29lap::pJB12; SmrApr This study
 JB123JB29 ΔpepA; Smr This study
 JB129JB123toxR::pVM55; SmrApr This study
 JB131JB123tcpP::pYM2-16; SmrApr This study
 JB132YM2-34 ΔpepA; Smr This study
 JB134JB29toxR::pVM55; SmrApr This study
 JB135JB29tcpP::pYM2-16; SmrApr This study
 JB148YM2-34aphB::pJB15; SmrApr This study
 JB150JB132aphB::pJB15; SmrApr This study
 JB152JB123 ΔpepA::pJB14; SmrApr This study
 YM2-34O395lacZ::tcpP-gusA; Smr 45
E. coli
 DH5αF endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA)U169 (φ80dlacZΔM15) 20
 DH5α λpir λpir lysogen of DH5αLaboratory strain
 SM10 λpir thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpirR6K 43
Plasmids
 pUJ10Plasmid for the construction of divergent fusions tolacZ/phoA reporters; Apr 12
 pGP704Suicide vector; pBR322 derivative with oriR6K mobRP4; Apr 19
 pT689A p6891MCS derivative which contains the rrnB transcriptional terminator from plasmid pKK223-3; Apr 45
 pCVD442Positive selection suicide vector; Apr 16
 pYM2-16pGP704 derivative carrying a 230-bp internaltcpP fragment; Apr Y. M. Murley and S. B. Calderwood, unpublished data
 pVM55Suicide plasmid pJM703.1, containing an internal fragment of toxR; Apr 43
 pJB4pUJ10 derivative carrying the toxT-gusA fusion; Apr This study
 pJB6pT689 derivative carrying the toxT-gusAfusion; Apr This study
 pJB10pGP704 derivative carrying an internal pepA fragment; Apr This study
 pJB12pGP704 derivative carrying an 800-bp internal fragment of lap; Apr This study
 pJB13pCVD442 derivative carrying a pepA allele containing an in-frame deletion; Apr This study
 pJB14pGP704 derivative carrying a wild-type copy ofpepA; Apr This study
 pJB15pGP704 derivative carrying a 584-bp internal fragment of aphB; Apr This study
  • a Sm, streptomycin; Ap, ampicillin; Km, kanamycin; Tc, tetracycline.