Table 3.

Activation from PhbpC and PhbpD in dependency of IHF and HU

StraindPlasmidseLuciferase activity (relative light units) ± SDawith:Induction factorc
No inducer2-HBPb
N99pHYBP103, pHYBP1311.08E+05 ± 7.70E+032.13E+06 ± 5.06E+0520
pHYBP106, pHYBP1311.26E+05 ± 1.53E+043.72E+05 ± 4.95E+042.9
A5475pHYBP103, pHYBP1316.22E+04 ± 9.73E+037.21E+04 ± 1.49E+041.2
pHYBP106, pHYBP1315.18E+04 ± 1.26E+044.96E+04 ± 1.71E+040.96
A5196pHYBP103, pHYBP1312.61E+04 ± 1.34E+035.56E+05 ± 4.88E+0421
pHYBP106, pHYBP1312.74E+04 ± 3.80E+034.06E+04 ± 4.54E+031.5
  • a All values were obtained in two independent experiments, each performed in triplicate.

  • b Assay concentration for 2-HBP was 0.2 mM.

  • c Induction factor is the ratio of the bioluminescence measured with and without the inducer.

  • d E. coli strains A5196 and A5475 are derivatives of E. coli N99 without HU protein and IHF, respectively.

  • e Plasmid pHYBP103 containshbpC::luxAB, pHYBP106 containshbpD::luxAB, and pHYBP131 containshbpR.