Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant featuresSource or reference
E. coli
 DH5supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1Takara
 JM109recA1 endA1 gyrA96 thi hsdR17 supE44 relA1Δ(lac-proAB)/F′ [traD36 proAB+lacIqlacZΔM15]Takara
 S17-1/λpirTra+recA pro thi hsdRchr::RP4-225
 XLI-BlueMR/pDPT51Δ(mcrA)183Δ(mcrCB-hsdSMR-mrr) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac(Con) pDPT51(Ampr)28
 BLR(DE3)pLysSFompT hsdSB(rBmB) gal dcmΔ(srl-recA)306::Tn10(Tcr)DE3pLysS(Cmr)Novagen
R. eutropha
 H16Wild typeATCC 17699
 D1phaZI::pJpPH171, KmrThis study
Plasmids
 pJDR215Wide-host-range cosmid, cloning vector; Kmr Smr3
 pMC1871Fusion vector; TcrAmersham
 pJP5603R6K-based suicide vector; Kmr17
 pUC18Cloning vector; AmprTakara
 pET23bExpression vector; AmprNovagen
 Charomid 9-36Cosmid; AmprNippon Gene
 pAE17pUC18 with R. eutropha phaZThis study
 pET171pET23b carryingXbaI/Bpu1102I fragment of pAE171This study
 pET171HpET23b carrying NdeI/XhoI fragment (1,263 bp) containing His-tagged phaZThis study
 pJPPH171pJP5603 carrying PstI/HincII fragment of pAE171This study
 pJDR215TpJDR215 carryingEcoT14/HindIII fragment of pMC1871 containing Tcr geneThis study
 pJDR171TpJDR215T carryingSnaBI/EcoRV fragment of pAE171 containingphaZThis study