Table 1.

Bacterial strains used in this work

StrainRelevant genotype or characteristicsaSource or reference
E. coli
 CC118λpir Host strain to propagate plasmids with an R6K origin of replication 17
 DH5αHost strain in routine cloning experimentsGibco BRL, Life Technologies
 N99 galK 28
 A5196Cmr Kmr; N99hupA16::Kan hupB11::Cat; derivative of strain N99 lacking the α and β subunits of HU 28
 A5475N99 ΔhimA82 hip157; derivative of strain N99 lacking the α and β subunits of IHF 28
 ET8000 rbs lacZ::IS1 gyrA hutC K(Con) 27
 ET8045Tcr; ET8000rpoN208::Tn10; derivative of strain ET8000 lacking the ς54 subunit 27
P. azelaica
 HBP12-HBP+; wild type 21
 HBP104Kmr; 2-HBP+; contains mini-Tn5 (region 1 [PhbpC]-luxAB res-npt-res) of plasmid pHYBP104 20
 HBP107Kmr; 2-HBP+; contains mini-Tn5 (region 2-luxAB res-npt-res) of plasmid pHYBP107This work
 HBP108Kmr; 2-HBP+; contains mini-Tn5 (region 3 [PhbpD]-luxAB res-npt-res) of plasmid pHYBP108This work
 HBP118Kmr; 2-HBP+; contains mini-Tn5 (region 4-luxAB res-npt-res) of plasmid pHYBP118This work
 HBP104121Kmr Smr Tcr; 2-HBP; derivative of strain HBP104 with disruptedhbpR 20
 HBP108121KmrSmrTcr; 2-HBP; derivative of strain HBP108 with disrupted hbpR This work
  • a 2-HBP+, ability to grow on 2-HBP; 2-HBP, inability to grow on 2-HBP;res-npt-res, element with kanamycin resistance gene of Tn5 (npt) flanked by tandem multimer resolution sequences (res) of RP4 (24); regions 1 to 4 correspond to the hbp-derived intergenic regions as shown in Fig. 1C.