Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Reference
Strains
S. mutans
  GS-5Wild type, serotype c 41
  BEE ahpC::Emr::nox-1 18
  DES dpr::Spcr This study
  KD251 sod::Emr This study
  BEE-DES dpr::Spcr ahpC::Emr::nox-1 This study
  KD251-DES dpr::Spcr sod::Emr This study
E. coli
  DH5α supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 14
  TA4315ΔahpC ΔahpF 36
Plasmids
 pUC118Apr lacPOZ 44
 pACYC184TetrCmr 6
 pSPC1Spcr1.1-kb EcoRI fragment 23
 pS235.3-kb fragment containing dpr in pUC118This study
 pS23B12.6-kbBamHI-BamHI fragment containing dprfrom pS23 in pUC118This study
 pS23E121.8-kbEcoRI-EcoRI fragment containing dprfrom pS23 in pUC118This study
 pS23E1210.94-kbEcoRI-EcoRI fragment from pS23E12This study
 pS23E1220.86-kb EcoRI-EcoRI fragment from pS23E12This study
 pD13184 dpr in pACYC184This study
 pS23B1ES dpr::Spcr from pS23B1This study
 pS23E12ES dpr::Spcr from pS23E12This study
 pKD251 sod::Emr 27