Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsReference
Strains
 C58Wild-type virulent strain containing nopaline Ti plasmid pTiC58 31
 NT1C58 cured of its Ti plasmid pTiC58 46
 NT1RERifr Emr mutant of NT1 46
 NT1REBFlagellum-free mutant of NT1RE 10
 A136Rifr, C58 cured of its Ti plasmid pTiC58 46
 Ach5Wild-type virulent strain containing octopine Ti plasmid pTiAch5 31
 LBA4011Rifr, Ach5 cured of its Ti plasmid pTiAch5 27
 A348Rifr, A136 containing octopine Ti plasmid pTiA6NC 20
 PC1001 to PC1011Rifr, ?virB1 to ?virB11 in pTiA6NC of A348, 11 virB nonpolar mutants 5
 Ax56Kanr,virB10::Tn5virB in pTiA6NC of A348,virB10 nonpolar mutant 6
 Ax42Kanr,virB9::Tn5virB in pTiA6NC of A348,virB9 nonpolar mutant 13
 Ax46Kanr,virB8::Tn5virB in pTiA6NC of A348,virB8 nonpolar mutant 13
 Ax68Kanr,virB6::Tn5virB in pTiA6NC of A348,virB6 nonpolar mutant 6
 Ax59Kanr,virB5::Tn5virB in pTiA6NC of A348,virB5 nonpolar mutant 6
 Ax79Kanr,virB4::Tn5virB in pTiA6NC of A348,virB4 nonpolar mutant 6
 368mxCarbr,virB11::Tn3HoHo1 in pTiA6NC of A348,virB11 mutant 42
 At12506Carbr,virD4::Tn3HoHo1 in pTiA6NC of A348,virD4 mutant 42
Plasmids
 pJK270Kanr, T-DNA::Tn5 in pTiC58Trac 26
 pJK107Kanr,virA::Tn5 in pTiC58Trac,virA mutant 33
 pJK104Kanr,virB5::Tn5 in pTiC58Trac,virB5 polar mutant 33
 pJK502Kanr,virB3::Tn5 in pTiC58Trac,virB3 polar mutant 33
 pJK195Kanr,virD1::Tn5 in pTiC58Trac,virD1 polar mutant 26
 pJK504Kanr,virD4::Tn5 in pTiC58Trac,virD4 mutant 21
 pJK130Kanr,virD2::nptII in pTiC58Trac,virD2 nonpolar mutant 32
 pJK734Kanr,virD4::nptII in pTiC58Trac,virD4 nonpolar mutant 32
 pUCD4606Kanr, virB2 nonpolar deletion mutant in pJK270 22