Table 3.

NBU2 ORFs and the characteristics of the putative proteinsa

ORFRange 5′–3′ (bp)% G+CLength (aa)Size (kDa)pISearch results and comments
linAN2 769–23740.517119.84.552% identity and 70–72% similarity to LinA′ from S. aureusBM4611 and LinA on pIP855 in S. haemolyticus (5,6)
mefEN2 2,000–80441.340344.39.133–34% identity and 52–54% similarity to MefE of S. pneumoniaeand MefA of S. pyogenes (8, 58)
attN2 2,000–2,400Two target sites: 13-bp identity to attN2 at the 3′ end of two Ser-tRNAUGA genes, followed by inverted repeats
intN2 2,499–3,78245.842750.48.926–28% identity and 44–49% similarity to the C-terminal ends of MTn5520 IntBIP and NBU1 IntN1 (46,60); member of the lambda family of site-specific integrases
orf2 3,846–6,19442.978391.35.426% identity and 45% similarity to an E. coli hypothetical protein
orf3 6,736–7,81546.336041.58.637% identity and 52% similarity to sigma 70 of Rhizobium sp.
prmN2 8,028–8,98448.131936.89.1>85% sequence identity to prmN1 of NBU1 and sequence on MTn4399 (20); 30–33% identity and 52–57% identity to the N-terminal ends of DNA primases from L. monocytogenes and B. subtilis dnaE.
oriTN2 880–910Gram-positive family of nick sites also shared by NBU1, MTn4555, pBI143 and pIP421 (53)
mobN2 9,137–10,54344.046955.19.388% nucleotide identity to NBU1 mobN1 and 84% nucleotide identity to MTn4555 mobATn ; 44, 48, and 55% aa sequence similarity to pIP421 Mob421, pBI143 MobA143, and MTn5520 MobBMP, respectively (20, 52, 60)
  • a aa, amino acids.