Table 2.

Primers used for PCR amplification

Purpose (size [bp])Primer, sequencea(5′ to 3′)
Joined ends of NBU2 (455) or to amplify the cloned chromosomal DNA between the ends (2,122–2,574)RN2end, CTT TCA GGA CGA TGT AAA GTC CTG
LN2end, GTA CGT CTC CTA CGA TTG GCA CAC
Target site 1 in BT4001 (466)TRN2, TCC AAG AGC AAC AAG TCA AGA TGC
TLN2, TTA GAG CGC ACA AAG TTA CAA TCA
intN2-attN2 (1,882); minimal integrative region and probe (7,420–8,391)IntFN2, TGT AAT TGC CTA TCT TCC AGT GAT G
IntRN2, AAC AAA TAC TTT CAG GAC GAT GTA A
ermG; erythromycin-clindamycin resistance marker for Bacteroides spp.ErmGF, GAA CAC CTG CAG 1 AAA AGT CGG GGA TTG GTG AAC
ErmGR, GAC AGA CTG CAG 1 ACA CCT TGT TAT TGG ACG CCT AC
prmN1 (974); NBU1 sequence (7,420–8,391).PrmN2F, CGC AAG ACC ATG G 2 CA ATA GAA GAA
PrmN2R, AGT GGGAGA TCT 3 CCG AAA GCC GTT TTT
  • a The underlined sequences are restriction sites inserted in the primers: 1, PstI; 2,NcoI; 3, BglII.