Table 4.

Summary of the results using NBU probes onBacteroides isolates

Probe% Bacteroides strains before 1970a%Bacteroides strains from 1980 to 1990sbSummary totals (n = 291)
Community (n = 75)Clinical (n = 23)Community (n = 105)Loyola, Va. clinicals (n = 65)Other clinicals (n = 26)
4.5-kbp HindIII (NBU1)c 48 (36)35 (8)75 (79)92 (60)91 (21)70 (204)
 IntN1176239203830
 IntN2223739475240
 IntN1 and IntN2d 63319102517
MObN1N2 248.755666950
PrmN1N2 281358747452
(MefE-LinA)N2 5.38.737433928
  • a Community and clinical isolates from the VPI anaerobe laboratory isolated in the 1960s and 1970s (16).

  • b The community isolates were isolated by participants in the Microbial Diversity Course at Woods Hole, Mass., in 1996 and 1997. The majority of the clinical isolates are from the VA Hospital at Loyola of Chicago Medical Center (65 isolates) and the Wadsworth Anaerobe Laboratory in Los Angeles, Calif. (14 isolates). The remaining isolates were requested and received from different hospitals and laboratories.

  • c Four ORFs from the C-terminal end ofintN1 through the N-terminal end of mobN1, including prmN and the oriT region (Fig. 2B). The percentages of the strains that hybridized to theHindIII fragment of NBU1 that also hybridized to theintN1 or intN2 probes are given. The numbers of strains tested are indicated in parentheses.

  • d A total of 17% of the strains hybridized to both IntN1 and IntN2, which means that the overall percentage of the strains that hybridized to the HindIII probe and the IntN in hybridization pattern was: 17% N1-N2 + 13% N1 only + 23% N2 only = 53%, or 108 of the 204 strains.