Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsReference or source
E. coli
 MV1184 araΔ(lac-proAB)rpsL thi (φ80 lacZΔM15) Δ(srl-recA)306::Tn10(Tcr) F′[traD36 proAB+ lacl q lacZΔM15] 23
 HB101 hsdS20 recA13 proA2 leu6 thi-1 rpsL20 ara-14 galK2 lacY1 xyl-5 mtl-1 supE44 3
P. aeruginosa
 PAO1Prototroph, FP (sex factor minus) 8
 PP1PAO1 derivative,ctpH::kan This study
 PP2PAO1 derivative,ctpL::kan This study
 PP3PAO1 derivative, ctpH::tet ctpL::kan This study
 APC1PAO1 derivative, phoU 12
 PHOB1PAO1 derivative,phoB::kan 12
 PP4PAO1 derivative, phoU ctpH::kan This study
 PP5PAO1 derivative, phoU ctpL::kan This study
 PP6PAO1 derivative,phoB::kan ctpH::tet This study
 PP7PAO1 derivative, phoB::kan ctpL::tet This study
Plasmids
 pGEM-T EasyPCR cloning vector; Apr lacPOZPromega
 pUC118TcpUC118 containing a 1.3-kb tet cartridge from pBR322; AprTcr This study
 pUC4KpUC4 containing a 1.3-kbkan cartridge; Apr Kmr Pharmacia
 pCP19Broad-host-range cosmid; TcrIncP 6
 pPT10.1pGEM-T Easy with a 3.6-kb PCR fragment containing ctpH; Apr This study
 pPT11.1pGEM-T Easy with a 3.3-kb PCR fragment containingctpL; Apr This study
 pPT10.2pPT10.1 derivative containing a 1.3-kb kan cartridgeThis study
 pPT11.2pPT11.1 derivative containing a 1.3-kb kancartridgeThis study
 pPT10.3pPT10.1 derivative containing a 1.3-kb tet cartridgeThis study
 pPT10.4pCP19 derivative containing a 3.6-kb EcoRI fragment of pPT10.1; Tcr This study
 pPT11.4pCP19 derivative containing a 2.3-kb EcoRV fragment of pPT11.1; Tcr This study
 pKZ27Broad-host-range transcriptional fusion vector; Kmr lacZIncQThis study
 pKZ27.1pKZ27 containing a 1.2-kb fragment with amp gene from pBR322; KmrCbr This study
 pPT10.5pKZ27 derivative containing a 1.8-kb KpnI fragment of pPT10.1; Kmr This study
 pPT10.6pKZ27.1 derivative containing a 1.8-kb KpnI fragment of pPT10.1; Kmr Cbr This study
 pPT11.5pKZ27 derivative containing a 1.6-kb EcoRV-SalI fragment of pPT10.1; Kmr This study
 pPT11.6pKZ27.1 derivative containing a 1.6-kbEcoRV-SalI fragment of pPT10.1; KmrCbr This study