Table 1.

Bacterial strains and plasmids

Strain or plasmidGenotype or propertyaSource or referenceb
Serovar Typhimurium
 CS015 phoP102::Tn10d-Cam 32
 CS401 phoN2 zxx-6251::Tn10d-Cam rpsL(Strr) 21
 CS09314028s; wild typeATCC
 CS022 pho-24 (PhoP constitutive); a mutation in phoQ that results in increased net phosphorylation of PhoP and unregulated expression of PhoP-activated genes (18) 33
 TG59CS401 ΔpgtE::KanThis work
 TG61CS022 ΔpgtE::KanThis work
 TG71TG61 with pBluescript KS+This work
 TG73TG61 with pTG73This work
 CS435 pho-24 pagP::Tn10d-TetphoN2 zxx-6251::Tn10d-Cam (also LG069) 21
 TG66CS435 ΔpgtE::KanThis work
 TG172CS401 with pTG171This work
 TG173CS015 with pTG171This work
 TG174CS022 with pTG171This work
 TG200CS022pagA::MudJThis work
 CS404CS022pagA::MudJpagP::Tn10d-TetThis work
E. coli
 SM10 λpir thi-1 thr-1 leuB6 supE44 tonA21 lacY1 recA::RP4-2-Tc::Mu
 DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 deoR thi-1 supE441 gyrA96 relA1 Gibco-BRL
 CC118 araD139Δ(ara,leu)7697 ΔlacX74 phoAΔ20 galE galK thi rpsE rpoB argE(Am)recA1 28
Plasmids and transposons
 pKAS32Ampr Strs (rpsL); a low-copy allelic exchange vector 41
 pTG51Ampr Kanr; pgtE with an inserted Kanr cassette (39) replacing a portion of pgtE is ligated toKpnI-SacI fragment of pKAS32This work
 pBluescript KS+Ampr; a high-copy cloning vectorStratagene
 pTG73Ampr; containspgtE and 904 bp of upstream region (nt 671 to 2671; GenBank accession no. M21279) cloned into XbaI site of pBluescript KS+This work
 pGPLFR03Ampr; firefly (f-luc) and vanilla (v-luc) double luciferase suicide vector 30
 pTG171Ampr; contains a transcriptional fusion ofpgtE (nt 5266 to 5641; GenBank no. M21279) tof-luc; ligated with an EcoRI-KpnI fragment of pGPLFR03This work
 pWSK129Kanr; low-copy cloning vector 51
 pTG82Kanr; contains pgtE and 904 bp of upstream region (nt 671 to 2671; GenBank no. M21279) cloned intoXbaI site of pWSK129This work
 pTG85Kanr; contains TnphoA/in insertion at codon 112 of pgtE in pTG82This work
 pTG86Kanr; contains TnphoA/in insertion at codon 152 of pgtE in pTG82This work
 pTG87Kanr; contains TnphoA/in insertion at codon 190 of pgtE in pTG82This work
 TnphoA/inTn5 derivative; generates translational fusions to alkaline phosphatase (phoA) 27
  • a nt, nucleotide.

  • b ATCC, American Type Culture Collection.