Table 4.

Expression of some amino acid biosynthetic operons and the PTS operon in an exponential-phase culture in minimal medium

OperonGeneappm (100)Rank
Histidine hisLb 3.3556
hisG 5.6265
hisD 2.5818
hisC 3.7490
hisB 3.3555
hisH 2.11,000
hisA 1.81,185
hisF 2.5824
hisI 4.2417
Isoleucine-valine ilvG 7.5171
ilvM 2.5827
ilvE 2.9668
ilvD 1.61,360
ilvA 0.63,038
IRcregulator ilvY∗∗ 0.43,373
IR ilvC 41.75
ALSd I ilvB 1.31,765
ilvN 1.71,295
ALS III ilvIb 2.9662
ilvH 3.0632
Leucine leuA 11.382
leuB 10.993
leuC 6.3215
leuD 9.6115
Threonine thrL 16.841
thrA 4.0442
thrB 5.4276
thrC 7.9160
Tryptophan trpL 6.6199
trpE 6.8189
trpD 4.3396
trpC 10.4102
trpB 11.677
trpA 13.264
Proline AB proA 2.5835
proB 2.4863
Proline C proC 1.11,994
Arginine E argE∗∗ 1.61,353
Arginine CBH argC 2.01,088
argB 2.9684
argH 2.2967
PTS ptsH 31.012
ptsI 19.630
crr 11.974
  • a Genes within operons are listed in the order in which they are transcribed (2), except for the divergent clusters, where the sites of initiation lie between the genes labeled with one and two asterisks.

  • b Multiple PCR products were derived from theilvI- and hisL-specific amplifications.

  • c IR, isomeroreductase.

  • d ALS, acetolactate synthase.