Table 1.

Strains, plasmids, and primers used in this study

Primer, strain, or plasmidGenotype, description, or sequenceaReference or source
Strains
E. coli
  DH5αφ80 Δ(lacZ)M15 Δ(argF-lac)U169 endA1 recA1 46
hsdR17(r K m K +) deoR thi-1 supE44 gyrA96 relA1
S. aureus
  8325-4Wild-type strain cured of prophagesLaboratory stock
  RN4220Restriction-deficient transformation recipientLaboratory stock
  MJH001 perR::kan Horsburghb
  MJH002 ahpC::pAZ106ahpC + Horsburgh
  MJH005 fur::pAZ106fur + Horsburgh
  MJH006 katA::pAZ106katA + Horsburgh
  MJH007 mrgA::pAZ106mrgA + Horsburgh
  MJH008 perR::pAZ106perR + Horsburgh
  MJH010 fur::tet This study
  MJH011 fhuD2::pAZ106fhuD2 + This study
  MJH012 sirA::pAZ106sirA + This study
  MJH013 orf4::pAZ106orf4 + This study
  MJH014 sstA::pAZ106sstA + This study
  MJH201 fur::tet perR::kan This study
  MJH211 fur::tet fhuD2::pAZ106 fhuD2 + This study
  MJH212 fur::tet sirA::pAZ106 sirA + This study
  MJH213 fur::tet orf4::pAZ106 orf4 + This study
  MJH214 fur::tet sstA::pAZ106 sstA+ This study
  MJH202 fur::tet ahpC::pAZ106 ahpC + This study
  MJH206 fur::tet katA::pAZ106 katA + This study
  MJH207 fur::tet mrgA::pAZ106 mrgA + This study
  MJH208 fur::tet perR::pAZ106 perR + This study
  MJH306 fur::tet perR::kan katA::pAZ106katA +
Plasmids
 pAZ106Promoterless lacZ erm insertion vector 59
 pAUL-ATemperature-sensitiveerm integrational shuttle vector 13
 pMAL14OL 23-24 fhuD2 PCR fragment in pAZ106 (1.1 kb)This study
 pMAL15OL27-28 sirA PCR fragment in pAZ106 (1.01 kb)This study
 pMAL20OL38-39orf4 PCR fragment in pAZ106 (0.75 kb)This study
 pMAL26OL51-26 sstA PCR fragment in pAZ106 (0.75 kb)This study
 pMAL17 EcoRI-XhoI PCR fragment containing a tet cassette (2 kb) inserted in aKpnI site engineered into the fur gene by PCRThis study
Primers
 OL22CCAGAATTCCGTAAGCACGTATAATTCCTTCTTGThis study
 OL23CACAGGATCCAACGATTGCAACATTGCCAACTGTGCThis study
 OL24CCAGAATTCGCTTGATATAATACTTCTCCACCThis study
 OL26CCAGAATTCGAAGTTGCAATGGCAGCACCTACThis study
 OL27CCAGAATTCCCACTACATCCTGCThis study
 OL28AATTGGATCCGGTACACGACTAGCACCGATThis study
 OL29AACCGCTCGAGTGATCGTTCAGAAGTGATTGCAGCThis study
 OL30CCGGTACCTTCCAACGATGTCCACTCCCCTACThis study
 OL31CCGGTACCCATGGTGTGTGTGAAACGTGCCAAThis study
 OL32CCGGTACCCGGATTTTATGACCGATGATGAAGThis study
 OL33CCGGTACCTTAGAAATCCCTTTGAGAATGTTTThis study
 OL38AATTGGATCCTATCTCTTCCTTGTAAAATCATCTCThis study
 OL39CCAGAATTCGGCATGTATCTTGATGCATCTTCAGThis study
 OL51CACAGGATCCATCTCATTGCGCACGAGTGCTGThis study
  • a Restriction enzyme sites used for cloning are underlined.

  • b Horsburgh et al., submitted.