Table 3.

Transcriptional analysis of R. capsulatuswild-type and mutant strains carrying an in-frameureDA-lacZ fusion (pNIRUB35)

Strain (pNIRUB35)Relevant genotypeβ-Galactosidase activity witha:
NH4+SerineUreaUrea plus NH4+Urea plus serine
B10SWild type434.4 ± 47.81,556.7 ± 109.11,529.1 ± 166.1448.3 ± 53.31,577.9 ± 373.5
TD50 ntrC 49.2 ± 4.731.6 ± 4.667.7 ± 9.062.8 ± 12.328.8 ± 6.6
KS111 ntrA 556.5 ± 25.53,369.4 ± 138.22,638.4 ± 287.9782.7 ± 124.62,412.1 ± 196.3
KS36ΔnifHDK 256.7 ± 15.91,471.0 ± 104.31,503.8 ± 59.1309.5 ± 41.71,787.9 ± 111.9
  • a β-Galactosidase activities (in Miller units) were determined in late-exponential-phase R. capsulatus cultures grown photoheterotrophically with the indicated N sources. Miller units (20) and standard deviations were calculated from three independent assays for each strain.