Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaReference or source
Bacterial strains
E. coli
  JM83Host for pUC plasmids 30
  S17-1RP4-2 (Tc::Mu) (Km::Tn7) integrated in the chromosome 29
 R. capsulatus
  B10SSpontaneous Smrmutant of R. capsulatus B10 14
  KS36ΔnifHDK::[Spc] 31
  KS111 ntrA::[Gm] 16
  TD50 ntrC::[Gm]T. Drepper, Bochum (1998)
  Xan-9, -10, -19, -20, -22Tn5-induced Ure mutants 17
Plasmids
 pUC18 lacZα Apr 30
 pPHU235Broad-host-range lacZfusion vector, Tcr 10
 pSUP202AprCmr Tcr mob 29
 pSUP301Apr Kmr mob 29
 pSUP401Cmr Kmr mob 29
 pNIRUB413.3-kb EcoRI fragment from Xan-10 (ureC::Tn5) in pUC18This work
 pNIRUB35 ureDA-lacZ fusion in pPHU235, Tcr This work
 pNIRUB42-I1.3-kbEcoRI fragment carrying the ureD promoter in pUC18This work
 pNIRUB46pSUP202 derivative carryinglrp2::[Gmr], Spcr This work
 pNIRUB5310-kb BamHI fragment of Xan-9 (ureF::Tn5) in pUC18, Kmr This work
 pNIRUB546-kb BamHI fragment of Xan-19 (ureC::Tn5) in pUC18, Kmr This work
 pNIRUB558-kbBamHI fragment of Xan20 (ntrC::Tn5) in pUC18, Kmr This work
 pNIRUB5610-kb BamHI fragment of Xan-22 (ntrB::Tn5) in pUC18, Kmr This work
 pNIRUB73-I/IIpSUP202 derivative carryingorf136::[Gmr], Kmr This work
 pNIRUB76-I/IIpSUP401 derivative carrying orf433::[Kmr], Gmr This work
 pBKRUB1-I/IIpSUP301 derivative carrying Δ(lrp1-lrp2-ureD)::[Gmr], Kmr This work
 pBKRUB15-IpSUP401 derivative carrying ureG::[Kmr], Gmr This work
 pBKRUB16/17pUC18 derivative carrying orf323::[Kmr], Tcr mob This work
  • a Abbreviations: Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Gmr, gentamicin resistance; Kmr, kanamycin resistance; Spcr, spectinomycin resistance; Smr, streptomycin resistance; Tcr, tetracycline resistance.