Table 1.

Plasmids used

Plasmid Description (antibiotic resistance) Source or reference
pPctc-bgaBDerivative of pMLK83 carrying actc-bgaB fusion (Kmr)W. Schumann
pPclpC-bgaB Derivative of pMLK83 carrying a clpC-bgaB fusion (Kmr) W. Schumann
pPlon-bgaB Derivative of pMLK83 carrying a lonA-bgaB fusion (Kmr) W. Schumann
pPdnaK-bgaB Derivative of pMLK83 carrying a dnaK-bgaB fusion (Kmr) 20
pDL2/pgroE-bgaB Derivative of pDL carrying a groEL-bgaB fusion (Cmr) 40
pHTΔctsR Derivative of pHT315 used in introducing ΔctsR (Cmr) 6
pJL74 Shuttle plasmid replicating in both E. coli and B. subtilis (Apr Spr) 16
pdnaK1/2Derivative of pJL74 used to generate ΔdnaK strains (Spr) This work
phrcA1/2 Derivative of pJL74 used to generate the polar ΔhrcA mutation (Spr) This work
phrcA-np Derivative of pJL74 used to generate the nonpolar ΔhrcA mutation (Spr) This work