Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsSource or reference
P. aeruginosa
 PAO1Wild type 10
 PAO-MW20 rpoS mutant of PAO1, Gmr This study
 PAO-R1ΔlasRderivative of PAO1, Tcr 9
 PAO-MW1ΔlasI ΔrhlI derivative of PAO1, Hgr Tcr 31
 PDO111 rhlR::Tn501 derivative of PAO1, Hgr 3
E. coli
 DH5αF φ80dlacZΔM15Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 deoR gyrA96 thi-1 relA1 supE44 26
 S17-1 thi pro hsdR recA RP4-2 (Tet::Mu) (Km::Tn7) 28
Plasmids
 pCR2.1TA cloning vector, Apr Invitrogen
 pEX1.8Broad-host-range expression vector, Apr 23
 pSUP102pACYC184 carrying mobRP4, CmrTcr 27
 pRK2013 tra + helper plasmid for triparental matings, Kmr 5
 pHRP315Ω Smr/Spr cassette, Apr 20
 pHRP309PromoterlesslacZ, Gmr 20
 pTL61T lacZ transcriptional fusion vector, Apr 17
 pBBR1MCS-5Broad-host-range vector, Gmr 13
 pRPOS-1pCR2.1 containing a 2.8-kb rpoS fragmentThis study
 pRPOS-GmpRPOS-1 with aacC1 interrupting therpoS gene, Gmr This study
 pMW102 rpoS::aacC1 on pSUP102, mobilizable rpoS knockout plasmid, Gmr This study
 pMW105pEX1.8 containing ptac-rpoS, Apr This study
 pMW301Broad-host-rangehcnAB-lacZ reporter, Apr Cbr This study
 pMW303Broad-host-range phzABC-lacZ reporter, Apr Cbr This study
 pMW304Broad-host-range rhlR-lacZ reporter, Gmr Spr This study
 pMW305Broad-host-range rhlI-lacZ reporter, Gmr Spr This study
 pTn5-GmpUTmini-Tn5 containing aacC1, Gmr This study
 pJBA24Cloning vector for creating reporter fusions on Tn5, Apr 1
 pMRP24-RPOSpJBA24 containing rpoS promoter, Apr This study
 pMW24-RPOS rpoS promoter-lacZ fusion vector, Apr This study
 pUTminiTn5-GmDelivery plasmid for mini-Tn5-Gm, AprGmr This study
 pTn5-RPOSpUTmini-Tn5carrying rpoS-lacZ from pMW24-RpoSThis study