Table 3.

Aerobic sterol uptake (low-copy vector)

StrainaGenotypeSterol uptakeb
463-1CWild type1
CJ2-A upc2-1 11.4
H1Bylr228c::LEU2 1.5
H1B (++)ylr228c::LEU2 1.2
KS122 (++)ylr228c::LEU2 upc2-1 9.1
KS119 (++)ylr228c::LEU2 upc2::URA3 1.5
  • a ++, Strain was transformed with the pRS313 plasmid containing the YLR228c point mutation.

  • b Sterol uptake is represented as the fold difference compared to the wild type from an experiment performed in triplicate.