Table 1.

Bacterial strains and plasmids used for complementation analyses

Strain or plasmidDescriptionSource, parent, and/or reference
Strains
V. cholerae
  O395N1Wild type
  VcΔXΔmotXO395N1, this study
  VcΔYΔmotYO395N1 (20)
  VcΔABΔpomABO395N1, this study
  VcΔGΔfliGO395N1 (20)
  VcΔXYABΔmotX ΔmotY ΔpomABO395N1, this study
  VcΔXYABGΔmotX ΔmotY ΔpomAB ΔfliGO395N1, this study
  HM-1Spontaneously hypermotileVcΔXYABG, pMotAB, pBAD-VcG
  HM-2Spontaneously hypermotileHM-1
  HM-3Spontaneously hypermotileHM-2
E. coli EcΔG(DFB225) ΔfliGD. Blair (22)
Plasmids
 pACYC184Camr, cloning vector
 pMotAB(pJZ19), E. coli fliG in pACYC184D. Blair (6)
 pBAD-24Ampr, cloning vector
 pBAD-VcGV. cholerae fliG in pBAD-24This study
 pBAD-EcGE. coli fliG in pBAD-24This study
 pBAD-FP1V. cholerae-E. coli chimeric fliG in pBAD-24This study
 pBAD-FP2E. coli-V. cholerae chimeric fliG in pBAD-24This study