Table 4.

Genetic transformation of comCDE mutant strains of S. mutans

StrainaTransformant/recipient ratiob
No SCSPSCSP
NG8 (wt)3.5 ± 5.2 × 10−5 3.9 ± 2.6 × 10−3
SMCC13.6 ± 5.0 × 10−7 6.3 ± 5.2 × 10−4
SMCD11.8 ± 3.1 × 10−7 2.5 ± 5.6 × 10−7
SMCE12.3 ± 4.0 × 10−7 1.6 ± 5.8 × 10−7
JH10055.2 ± 4.2 × 10−7 8.8 ± 6.0 × 10−4
NG8(pVA-GTFA)4.2 ± 3.6 × 10−4 2.9 ± 2.5 × 10−2
  • a wt, wild type. The donor DNA used for transformation was replicative plasmid pDL289 carrying a kanamycin resistance gene except with NG8, which was also transformed with the integrative plasmid pVA-GTFA.

  • b All assays were performed with a saturating concentration of DNA (>1 μg/ml). The results are expressed as the mean value from three separate experiments ± standard deviation.