Genetic transformation of comCDE mutant strains of S. mutans
| Straina | Transformant/recipient ratiob | |
|---|---|---|
| No SCSP | SCSP | |
| NG8 (wt) | 3.5 ± 5.2 × 10−5 | 3.9 ± 2.6 × 10−3 |
| SMCC1 | 3.6 ± 5.0 × 10−7 | 6.3 ± 5.2 × 10−4 |
| SMCD1 | 1.8 ± 3.1 × 10−7 | 2.5 ± 5.6 × 10−7 |
| SMCE1 | 2.3 ± 4.0 × 10−7 | 1.6 ± 5.8 × 10−7 |
| JH1005 | 5.2 ± 4.2 × 10−7 | 8.8 ± 6.0 × 10−4 |
| NG8(pVA-GTFA) | 4.2 ± 3.6 × 10−4 | 2.9 ± 2.5 × 10−2 |
↵a wt, wild type. The donor DNA used for transformation was replicative plasmid pDL289 carrying a kanamycin resistance gene except with NG8, which was also transformed with the integrative plasmid pVA-GTFA.
↵b All assays were performed with a saturating concentration of DNA (>1 μg/ml). The results are expressed as the mean value from three separate experiments ± standard deviation.