Table 1.

Effect of metabolic inhibitors on glutamine transport by starved cells of S. lugdunensisa

AdditionGlutamine transport (nmol/mg of protein/10 min)
None 4.4
Glucose41
 +1 mM iodoacetate 8.0
 +5 mM KCN 20
 +10 μM CCCP 2.0
 +50 μM DCCD 54.0
Lactate 22
 +1 mM iodoacetate 23
 +5 mM KCN 4.4
 +10 μM CCCP 2.3
 +50 μM DCCD 66
  • a C. amycolatum could not be starved of metabolizable reserves by using 2,4-dinitrophenol alone. In contrast, the starvation protocol was equally effective for S. lugdunensis and C. amycolatum when 2,4-dinitrophenol was used in combination with valinomycin, as in Fig. 1D.