Table 1.

S. cerevisiae strains used in this study

StrainaGenotypebSource or reference
463-1C MAT a leu2 his3 trp1 ura3 K. Tatchelc
463-1D MATαleu2 his3 trp1 ura3 K. Tatchel
463-1C/463-1D MAT a/MATαleu2/leu2 his3/his3 trp1/trp1 ura3/ura3 K. Tatchel
CJ2-A MAT a upc2-1 leu2 his3 trp1 ura3 4
CJ625 MAT a upc2::URA3 leu2 his3 trp1 ura3 4
H1B MATα ylr228c::LEU2 leu2 his3 trp1 ura3 This study
H1B (+) MATα ylr228c::LEU2 leu2 his3 trp1 ura3 This study
KS93 MAT a upc2-1ylr228c::LEU2 leu2 his3 trp1 ura3 This study
KS93 (+) MAT a upc2-1ylr228c::LEU2 leu2 his3 trp1 ura3 This study
KS94 (+) MAT a upc2::URA3ylr228c::LEU2 leu2 his3 trp1 ura3 This study
H1B (++) MATα ylr228c::LEU2 leu2 his3 trp1 ura3 This study
KS122 (++) MAT a upc2-1 ylr228c::LEU2 leu2 his3 trp1 ura3 This study
KS119 (++) MAT a upc2::URA3ylr228c::LEU2 leu2 his3 trp1 ura3 This study
  • a +, Strain is transformed with the plasmid YEp351 containing the YLR228c point mutation; ++, strain is transformed with the plasmid pRS313 containing the YLR228c point mutation.

  • b upc2  = YDR213w.

  • c Kelly Tatchel, Louisiana State University Medical Center, Shreveport, La.