Table 1.

ADP-glucose pyrophosphorylase activity and starch accumulation in 137C (wild-type reference), NV314 (sta1-1), BAFJ5 (sta6-1::ARG7), and DP1 (vegetative diploid generated from fusion between NV314 and BAFJ5) strains

StrainsActivity (10−2)aActivation factorbStarch amtc
137C12.8 ± 3.4845 ± 8
NV3141.8 ± 0.712.5 ± 1.3
BAFJ5NDd NDND
DP110.7 ± 2.31055 ± 7
  • a The assay was performed in the degradation direction by measuring the glucose-1-phosphate formed in the presence of 1 mM ADP-glucose, 1 mM pyrophosphate and 1.5 mM activator 3-PGA. The activity is expressed in nanomoles of glucose-1-phosphate formed per milligram of protein per minute.

  • b The activation factor of ADP-glucose pyrophosphorylase represents the ratio between the activities measured with 1.5 mM 3-PGA and without 3-PGA.

  • c Amount of starch expressed (in micrograms) per 106 cells. Cells were grown in a nitrogen-deprived medium that allows a maximum accumulation of starch.

  • d ND, not detectable.